Ebright et al. 2020 (PRJNA601135)

General Details

Title	Deregulation of ribosomal protein expression and translation promotes breast cancer metastasis [ribo-Seq BRx142]
Organism	Homo sapiens
Number of Samples	16
Release Date	2020/01/14 00:00
Sequencing Types
Protocol Details

Repository Details

SRA	SRP241899
ENA	SRP241899
GEO
BioProject	PRJNA601135

Publication

Title
Authors	Ebright RY,Lee S,Wittner BS,Niederhoffer KL,Nicholson BT,Bardia A,Truesdell S,Wiley DF,Wesley B,Li S,Mai A,Aceto N,Vincent-Jordan N,Szabolcs A,Chirn B,Kreuzer J,Comaills V,Kalinich M,Haas W,Ting DT,Toner M,Vasudevan S,Haber DA,Maheswaran S,Micalizzi DS
Journal	Science (New York, N.Y.)
Publication Date	2020 Mar 27
Abstract	Circulating tumor cells (CTCs) are shed into the bloodstream from primary tumors, but only a small subset of these cells generates metastases. We conducted an in vivo genome-wide CRISPR activation screen in CTCs from breast cancer patients to identify genes that promote distant metastasis in mice. Genes coding for ribosomal proteins and regulators of translation were enriched in this screen. Overexpression of RPL15 , which encodes a component of the large ribosomal subunit, increased metastatic growth in multiple organs and selectively enhanced translation of other ribosomal proteins and cell cycle regulators. RNA sequencing of freshly isolated CTCs from breast cancer patients revealed a subset with strong ribosome and protein synthesis signatures; these CTCs expressed proliferation and epithelial markers and correlated with poor clinical outcome. Therapies targeting this aggressive subset of CTCs may merit exploration as potential suppressors of metastatic progression. Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
PMC	PMC7307008
PMID	32029688
DOI

	Run Accession	Study Accession	Scientific Name	Description	Cell Line	Library Type	Treatment	Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883784		Homo sapiens	circulating tumor cell - derived cell line				Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883785		Homo sapiens	circulating tumor cell - derived cell line				Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883786		Homo sapiens	circulating tumor cell - derived cell line		Ribo-Seq		Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883787		Homo sapiens	circulating tumor cell - derived cell line		Ribo-Seq		Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883788		Homo sapiens	circulating tumor cell - derived cell line		Ribo-Seq		Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883789		Homo sapiens	circulating tumor cell - derived cell line		Ribo-Seq		Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883790		Homo sapiens	circulating tumor cell - derived cell line		Ribo-Seq		Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883791		Homo sapiens	circulating tumor cell - derived cell line		Ribo-Seq		Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883792		Homo sapiens	circulating tumor cell - derived cell line		Ribo-Seq		Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883793		Homo sapiens	circulating tumor cell - derived cell line		Ribo-Seq		Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883794		Homo sapiens	circulating tumor cell - derived cell line		Ribo-Seq		Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883795		Homo sapiens	circulating tumor cell - derived cell line		Ribo-Seq		Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883796		Homo sapiens	circulating tumor cell - derived cell line		Ribo-Seq		Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883797		Homo sapiens	circulating tumor cell - derived cell line		Ribo-Seq		Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883798		Homo sapiens	circulating tumor cell - derived cell line		tRNA		Trips-Viz	GWIPS-viz	RiboCrypt
	SRR10883799		Homo sapiens	circulating tumor cell - derived cell line		tRNA		Trips-Viz	GWIPS-viz	RiboCrypt
	Run Accession	Study Accession	Scientific Name	Description	Cell Line	Library Type	Treatment	Trips-Viz	GWIPS-viz	RiboCrypt